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 | Volume 7, Number 1 (6-2009) |  | |
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 | The role of Rad51 protein in radioresistance of spheroid model of DU145 prostate carcinoma cell line |  |
| | Author(s): M. Taghizadeh, S. Khoei, A.R. Nikoofar, L. Ghamsari and B. Goliaei * | | Laboratory of Biophysics and Molecular Biology, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran goliaei@ibb.ut.ac.ir | | Study Type: Original Research | Subject: Radiation Biology | | | Article abstract: | | Background: Rad51 is a protein with critical role
in double strand break repair. Down-regulation of this
protein has a significant effect in radiosensitivity of
some cell lines like prostate carcinoma. Compared to
monolayer cell culture model, the spheroids are more
resistant to radiation. The aim of the current study
was to determine the Rad51 protein level in DU145
spheroids, and monolayer cells before and after
exposure to gamma irradiation. Materials and
Methods: In the present study, western blot was used
to determine the level of Rad51 protein in DU145 cell
line grown as monolayer and spheroid. Results:
Western blot analysis showed that in the spheroid
cells, Rad51 had an elevated level before and after
radiation in comparison with monolayer cells. Higher
doses of radiation induced elevated expression of
Rad51 protein in both culture models. The level of at
protein after exposure to gamma rays had been timedependent.
Conclusion: Rad51 might act as a
mediator of radiation resistance in tumor cells.
Repression of Rad51 activity could be a prominent
strategy to overcome radiation resistance of tumors.
Iran. J. Radiat. Res., 2009; 7 (1): 19-25 | | | Keywords: Multicellular spheroid, radioresistance, DU145 prostate carcinoma cell line, rad51., | |
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